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Antagen’s Lentivirus Titration XpressCard is a rapid lateral flow immunoassay that detects lentiviral capsule protein released into the packaging supernatant or from the purified viral stock, as a surrogate marker to determine whether virus production yields a usable titer for subsequent infection. This assay takes only 10-15 minutes, whereas the conventional reinfection based assay normally takes 2-3 days and requires FACS or fluorescent microscopy.
Antagen’s LentiTitre Cassette is a multi-lane lentivirus titration kit for rapid and accurate estimation of the functional lentivirus titres in less than 10 minutes. Each pouch contains one 4x lane casssette. It is a lateral flow immunoassay (LFIA) based titration device, based on p24 detection with sensitive gold-conjugated antibody. Lentiviral supernatant standards are included in the kit. The cassette can help accurately estimate lentiviral TU titres, using p24 as a gauge. It can also be used to determine the optimal time when packaging preparation is ready for harvesting.
The Rapid Mouse Immunoglobulin Isotyping XpressCard is a 5-min lateral flow assay with ELISA sensitivity. Acceptable sample types include hybridoma culture supernatant or purified Ig.
Each sealed pouch contains two cassettes.
ISO-M6 XpressCard detects IgG1, IgG2a and IgG2b in one cassette, and IgM, κ, and λ in the other.
ISO-M8a XpressCard detects 6 Ig heavy chain subtypes and 2 light chain types: IgG1, IgG2a, IgG2b and IgG3 in one cassette, and κ, λ, IgA and IgM in the other.
While BALB/c mice express IgG2a, some other inbred mouse strains such as C57BL/6, C57BL/10 and NOD do not have the gene for IgG2a and instead express the IgG2c isotype.
ISO-M8c XpressCard detects 6 Ig heavy chain subtypes and 2 light chain types (IgG1, IgG2b, IgG2c and IgG3 in one cassette, and κ, λ, IgA and IgM ) in the other.
ISO-M8ac XpressCard combines the essence of ISO-M8a and ISO-M8c and detects IgG1, IgG2a, IgG2b and IgG2c in one cassette, and IgG3, IgM, κ and λ in the other.
The Rapid Rat Immunoglobulin Isotyping XpressCard is a 5-min lateral flow assay with ELISA sensitivity. Acceptable sample types include hybridoma culture supernatant or purified Ig.
Each sealed pouch contains two cassettes. ISO-R8 XpressCard detects IgG1, IgG2a, IgG2b and IgG2c in one cassette, and IgM, IgA, κ, and λ in the other.
The Rapid Hamster Immunoglobulin Isotyping XpressCard is a 5-min lateral flow assay with ELISA sensitivity. Acceptable sample types include hybridoma culture supernatant or purified Ig.
Each sealed pouch contains two cassettes. ISO-HAM XpressCard detects IgG1 and κ of Armenian and Syrian strains, IgG2 and/or IgG3 of Armenian strain, and IgG2 of Syrian strain only.
The Rapid Human NGAL XpressCard is involved in innate immunity by sequestrating iron that in turn limits bacterial growth. It is expressed in neutrophils and in low levels in the kidney, prostate, and epithelia of the respiratory and alimentary tracts.
In the case of acute kidney injury (AKI), NGAL is secreted in high levels into the blood and urine within 2 hours of injury. NGAL levels in patients with AKI have been associated with the severity of their prognosis and can be used as a biomarker for AKI. NGAL can also be used as an early diagnosis for procedures such as chronic kidney disease, contrast induced nephropathy, and kidney transplant.
Fetal fibronectin (fFN) is a fibronectin protein produced by fetal cells and certain types of tumor cells. Fetal fibronectin “leaks” into the vagina if a preterm delivery is likely to occur and can be measured in a screening test. When the fFN test is positive, it is an inconclusive result. A positive result can indicate that a woman will go into preterm labor soon, but she may not go into labor for weeks. When the fFN test is negative, the result is a better predictor. A negative result means that there is little possibility of preterm labour within the next 7 to 10 days, and the test can be repeated weekly for women who remain at high risk. A negative fetal fibronectin test gives a more than 95% likelihood of remaining undelivered for the next 2 weeks.
fFN is also secreted into the urine of patients with bladder cancer (transitional cell carcinoma), and can be detected in the tissue culture supernatants of liver cancer, breast cancer and lung cancer cell lines. The possible onco-fetal relation of fFN gene regulation suggests that fFN could be an early biomarker for certain cancers.
Antagen’s hIL-8 XpressCard is an immunochromatographic rapid test for the detection of human interleukin-8 in biological fluids, such as urine, serum or plasma. The assay can be potentially used as an aid for diagnosis of primary and secondary infections (e.g., urinary tract infection and neonatal sepsis), as well as certain neoplastic conditions (e.g., bladder cancer), in conjunction with other criteria.
We offer two types of ELISA substrate 3, 3′, 5, 5′ tetramethyl benzidine (TMB), regular class and ultra class. Ultra class has a greater sensitivity than Regular class. The solutions A and B of each class should be mixed 1:1 just before use. We offer this quality at an inexpensive price.
This single buffer is to quickly lyse mouse tail/ear tissues to extract gDNA in just 30 minutes at boiling temerature, an INEXPENSIVE, CONVENIENT, and FAST alternative protocol over the conventional Proteinase K based method. 1 μl gDNA containing solution can be directly used in a 20 μl PCR reaction for mouse genotyping.
These cell lines can be used as control for fluorescent protein (GFP or DsRed) or in binding assay for certain antigens in FACS.
The Benchtop Iceman-Racks are made of metal with thermal superconductivity and make realtime changes (3-5 seconds) in temperature that fits your needs, ranging from -80ºC to +100ºC and is corrosion-proof. The tubes for them are ranging PCR tubes/plate to eppendorf tubes.