Add 100 µl of hamster antibody solution at appropriate concentration into the sample well. Within 5 minutes, test lines (T) will appear showing the isotype of the antibody. A control line (C) will appear at the far end, showing a successful run. This assay is faster and more convenient than the traditional ELISA method.
Order ISO-HAM to determine IgG1, IgG2*, IgG3, and κ.
* Unlike in the cases for mouse and rat antibodies, reagents specifically recognizing hamster antibody isotypes are not very common. The available IgG2-recognizing antibody also cross-reacts to IgG3 of the Armenian strain (for more info on strain-specific reactivity, refer to the manual below). Due to the lack of specific reagents, possible IgM and λ chains can only be inferred by the absence of IgG and κ signals.
Manual >>Rapid Hamster Antibody Isotyping Kit