Protein Expression Systems

Antagen Technology — Protein Expression System

Antagen has pioneered the biologics production through its innovative fast-track and high-efficiency protein expression system. The expression system not only shortens the development time of stable cell lines to 2-3 months from traditional 6-12 months, but also increases the expression yield of target proteins more than two dozenfold.

 Antagen Genetic Elements

Scientists at Antagen Pharmaceuticals have developed proprietary Antagen Genetic Elements (AGE) that were discovered with a novel bioinformatics tool and are now utilized to control the dynamic organization of transgenes in the chromatin.

Key Advantages


High Speed: Create a stable, high-productivity cell line with only 8-12 weeks
High targeting efficiency: Reduce the workload of screening and subcloning for establishing cell lines
High Yield: Protein expression levels at rates typically ranging from 60 to 100 p/c/d/
High Stability: Antagen Genetic Elements prevents the epigenetic silencing effect; integration into a ‘hot spot’ further safeguards the cell line stability
High Functionality: The compact design of Antagen expression vectors ensures both the simultaneous expression of multiple gene products and the stringent selection of high producers

Antagen Expression Vectors


Antagen Expression Vectors make multi-fold increase in protein expression level over the traditional. Meanwhile, the vectors’ compact design ensures both the simultaneous expression of multiple gene products and the stringent selection of high producers

Fast-Track Development of Stable Cell Lines


Antagen’s unique vector design and selection process greatly shorten the development time of stable CHO cell lines to 4-5 weeks. Meanwhile, a new expression system based on human cell line is under development at Antagen.

Process Development and Purification System


Antagen’s experts with the breadth of experience in protein process development, purification & characterization, and formulation ensure the products’ quality and minimize the processing time.