Add 100 µl of mouse antibody solution at appropriate concentration into the sample well. Within 5 minutes, a test line (T) will appear showing the isotype of the antibody. A control line (C) will appear at the far end, showing a successful run. This assay is faster and more convenient than the traditional ELISA method.
While BALB/c mice express IgG2a, some other inbred mouse strains such as C57BL/6, C57BL/10 and NOD do not have the gene for IgG2a and instead express the IgG2c isotype.
Order ISO-M8a to determine IgG1, IgG2b, IgG2c, IgG3, IgA, IgM, κ, and λ:
20 tests ($16.50 a test) Cat# ISO-M8c-20
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