The Rapid Mouse Immunoglobulin Isotyping Kit is a five-minute lateral flow assay with ELISA sensitivity for monoclonal antibody class and subclass determination. Acceptable sample types include hybridoma culture supernatant and purified monoclonal antibody. Ascites fluid is not recommended since it usually contains multiple immunoglobulins. However, this kit can still be used to determine the relative distribution of the main isotypes in a sample of ascites fluid.
Add 100 µl of mouse antibody solution at appropriate concentration into the sample well. Within 5 minutes, a test line (T) will appear showing the isotype of the antibody. A control line (C) will appear at the far end, showing a successful run. This assay is faster and more convenient than the traditional ELISA method.
The isotyping kit has three different sizes with 5, 10 or 20 pouches containing two cassettes per pouch: one cassette is for determining IgG1, IgG2a, IgG2b and IgG2c, and the other cassette is for determining IgG3, IgM, κ and λ. Both strips contain a control line “C”.
While BALB/c mice express IgG2a, some other inbred mouse strains such as C57BL/6, C57BL/10 and NOD do not have the gene for IgG2a and instead express the IgG2c isotype. For reference, read publications “Possible allelic structure of IgG2a and IgG2c in mice” by Zhang et. al, Molecular Immunology, 50 (2012) 169-171 and “The need for IgG2c specific antiserum when isotyping antibodies from C57BLr6 and NOD mice” by Martin et. al, Journal of Immunological Methods 212 (1998). 187–192.
Order ISO-M8ac to determine IgG1, IgG2a, IgG2b, IgG2c, IgG3, IgM, κ and λ.
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Manual >>Rapid Mouse Immunoglobulin Isotyping Kit – ISO-M8ac
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